Hotspot

psbA-matK

LSC · 1426-1956 · 531 bp

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Pi 0
MarkerSeek score 61.90
Primer available yes
Label psbA-matK

Feature Metadata

label_name
psbA-matK
feature_type
igs
region
LSC
length_bp
531
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-matK_p1 AAGACGGTTTTCGGTGCTAG CCCAAAAGCCTCTTCCACTT 50.0 50.0 58.2 58.0 683-718 bp 1.0 82.3
psbA-matK_p2 AAGACGGTTTTCGGTGCTAG TTCCCAAAAGCCTCTTCCAC 50.0 50.0 58.2 58.0 685-720 bp 1.0 82.3
psbA-matK_p3 AAGACGGTTTTCGGTGCTAG TTGACCTTCCCAAAAGCCTC 50.0 50.0 58.2 58.0 691-726 bp 1.0 82.3
psbA-matK_p4 AAAGACGGTTTTCGGTGCTA CCCAAAAGCCTCTTCCACTT 45.0 50.0 57.8 58.0 684-719 bp 1.0 82.0
psbA-matK_p5 TAAAGACGGTTTTCGGTGCT CCCAAAAGCCTCTTCCACTT 45.0 50.0 57.8 58.0 685-720 bp 1.0 82.0