Hotspot

ndhF-rpl32

SSC · 110082-110569 · 488 bp

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Pi 0.0329
MarkerSeek score 75.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
488
variable_sites
41
pi
0.0329
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACGAAAGGTAAAATACACGCA TGATGCAGAAGTACGTTTTT 38.1 35.0 56.2 53.5 560-578 bp 1.0 49.8
ndhF-rpl32_p2 ACGAAAGGTAAAATACACGCA TTGATGCAGAAGTACGTTTT 38.1 35.0 56.2 53.5 561-579 bp 1.0 49.8
ndhF-rpl32_p3 ACGAAAGGTAAAATACACGCA TTTGATGCAGAAGTACGTTT 38.1 35.0 56.2 53.5 562-580 bp 1.0 49.8
ndhF-rpl32_p4 ACGAAAGGTAAAATACACGCA TTTTGATGCAGAAGTACGTT 38.1 35.0 56.2 53.5 563-581 bp 1.0 49.8
ndhF-rpl32_p5 ACGAAAGGTAAAATACACGCA TTTTTGATGCAGAAGTACGT 38.1 35.0 56.2 53.5 564-582 bp 1.0 49.8