Hotspot

rps8-rpl14

LSC · 78949-79108 · 160 bp

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Pi 0.0358
MarkerSeek score 67.30
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
160
variable_sites
12
pi
0.0358
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 CAGCAATAGTGTCTCTACCCA GTTTCATTAGCTCCCGAGGT 47.6 50.0 56.8 57.3 199-216 bp 1.0 65.6
rps8-rpl14_p2 TCAGCAATAGTGTCTCTACCCA GTTTCATTAGCTCCCGAGGT 45.5 50.0 58.3 57.3 200-217 bp 1.0 64.7
rps8-rpl14_p3 CTCAGCAATAGTGTCTCTACCC GTTTCATTAGCTCCCGAGGT 50.0 50.0 57.7 57.3 201-218 bp 1.0 64.5
rps8-rpl14_p4 TCAGCAATAGTGTCTCTACCC GTTTCATTAGCTCCCGAGGT 47.6 50.0 56.5 57.3 200-217 bp 1.0 63.4
rps8-rpl14_p5 CAGCAATAGTGTCTCTACCCA AGTTTCATTAGCTCCCGAGG 47.6 50.0 56.8 57.0 200-217 bp 1.0 63.3