Hotspot

psbE-petL

LSC · 63757-64686 · 930 bp

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Pi 0.0254
MarkerSeek score 65.10
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
930
variable_sites
60
pi
0.0254
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 GTTCTCCTGTGCTTCCAGAC AGCTACTAGTAGAAAACCGA 55.0 40.0 58.2 52.8 984-998 bp 1.0 46.4
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC AGCTACTAGTAGAAAACCGAA 55.0 38.1 58.2 53.8 984-998 bp 1.0 46.4
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC AGCTACTAGTAGAAAACCGA 50.0 40.0 57.8 52.8 989-1003 bp 1.0 46.3
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC AAGCTACTAGTAGAAAACCGA 50.0 38.1 57.8 53.8 990-1004 bp 1.0 46.3
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC AGCTACTAGTAGAAAACCGAA 50.0 38.1 57.8 53.8 989-1003 bp 1.0 46.3