Hotspot

ndhC-trnV(UAC)

LSC · 49368-50267 · 900 bp

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Pi 0.0285
MarkerSeek score 68.30
Primer available yes
Label ndhC-trnV(UAC)

Feature Metadata

label_name
ndhC-trnV(UAC)
feature_type
igs
region
LSC
length_bp
900
variable_sites
62
pi
0.0285
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV(UAC)_p1 TCATATTCGTAAAGCAAAAACA GTTCGAGTCCGTATAGCCCT 27.3 55.0 52.7 58.7 921-990 bp 1.0 49.2
ndhC-trnV(UAC)_p2 TCATATTCGTAAAGCAAAAACA TTCGAGTCCGTATAGCCCTA 27.3 50.0 52.7 57.1 920-989 bp 1.0 49.2
ndhC-trnV(UAC)_p3 ATCATATTCGTAAAGCAAAAACA GTTCGAGTCCGTATAGCCCT 26.1 55.0 53.3 58.7 922-991 bp 1.0 49.2
ndhC-trnV(UAC)_p4 TCATATTCGTAAAGCAAAAACAT GTTCGAGTCCGTATAGCCCT 26.1 55.0 53.3 58.7 921-990 bp 1.0 49.2
ndhC-trnV(UAC)_p5 TCATATTCGTAAAGCAAAAACAT TTCGAGTCCGTATAGCCCTA 26.1 50.0 53.3 57.1 920-989 bp 1.0 49.2