Hotspot

trnG(GCC)-trnfM(CAU)

LSC · 35858-35995 · 138 bp

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Pi 0.0383
MarkerSeek score 70.80
Primer available yes
Label trnG(GCC)-trnfM(CAU)

Feature Metadata

label_name
trnG(GCC)-trnfM(CAU)
feature_type
igs
region
LSC
length_bp
138
variable_sites
14
pi
0.0383
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG(GCC)-trnfM(CAU)_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 203-211 bp 1.0 85.8
trnG(GCC)-trnfM(CAU)_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 204-212 bp 1.0 85.8
trnG(GCC)-trnfM(CAU)_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 239-247 bp 1.0 84.9
trnG(GCC)-trnfM(CAU)_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 240-248 bp 1.0 84.9
trnG(GCC)-trnfM(CAU)_p5 ATGCGGATATGGTCGAATGG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.9 58.0 280-288 bp 1.0 83.8