Hotspot

rpoB-trnC(GCA)

LSC · 26770-27876 · 1107 bp

Back to Lamium

Pi 0.0249
MarkerSeek score 66.00
Primer available yes
Label rpoB-trnC(GCA)

Feature Metadata

label_name
rpoB-trnC(GCA)
feature_type
igs
region
LSC
length_bp
1107
variable_sites
75
pi
0.0249
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC(GCA)_p1 GACATTGCCTCGTTTGCATC CCGGATTTGAACTGGGGAAA 50.0 50.0 58.1 58.1 1197-1238 bp 1.0 84.1
rpoB-trnC(GCA)_p2 TAGACATTGCCTCGTTTGCA CCGGATTTGAACTGGGGAAA 45.0 50.0 57.8 58.1 1199-1240 bp 1.0 83.5
rpoB-trnC(GCA)_p3 GACATTGCCTCGTTTGCATC TTTGGTAATCAGGCGACACC 50.0 50.0 58.1 58.2 1216-1257 bp 1.0 83.2
rpoB-trnC(GCA)_p4 TAGACATTGCCTCGTTTGCA TTTGGTAATCAGGCGACACC 45.0 50.0 57.8 58.2 1218-1259 bp 1.0 82.6
rpoB-trnC(GCA)_p5 GACATTGCCTCGTTTGCATC AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.1 58.4 1179-1220 bp 1.0 82.0