Hotspot

psaC

Genome · 72850-73095 · 246 bp

Back to Laminaria

Pi 0.0122

MarkerSeek score 45.90

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: Genome
length_bp: 246
variable_sites: 3
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	ACTAATTGGGTCACCTGGGA	AGGAGGTGCTGCGATAAAAG	50.0	50.0	57.9	58.0	1000 bp	1.0	81.1
psaC_p2	ACTAATTGGGTCACCTGGGA	TGTAACAAGAAGCCGCTGTT	50.0	45.0	57.9	58.0	974 bp	1.0	81.1
psaC_p3	TAATTGGGTCACCTGGGACT	AGGAGGTGCTGCGATAAAAG	50.0	50.0	57.9	58.0	998 bp	1.0	81.0
psaC_p4	TAATTGGGTCACCTGGGACT	TGTAACAAGAAGCCGCTGTT	50.0	45.0	57.9	58.0	972 bp	1.0	81.0
psaC_p5	GACCTTTACGTCGTGCTCTT	AGGAGGTGCTGCGATAAAAG	50.0	50.0	57.9	58.0	1438-1445 bp	1.0	80.8