Hotspot

F6D16_pgt018-rns

Genome · 81868-82317 · 450 bp

Back to Laminaria

Pi 0.0356

MarkerSeek score 68.10

Primer available yes

Label F6D16_pgt018-rns

Feature Metadata

label_name: F6D16_pgt018-rns
feature_type: igs
region: Genome
length_bp: 450
variable_sites: 16
pi: 0.0356
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
F6D16_pgt018-rns_p1	AATACGAACGTGGCGGAATT	GCATGTGTAAAGCATACCGC	45.0	50.0	58.3	57.9	586 bp	1.0	79.2
F6D16_pgt018-rns_p2	AATACGAACGTGGCGGAATT	TTCGTACGACTTGCATGTGT	45.0	45.0	58.3	57.9	598 bp	1.0	78.8
F6D16_pgt018-rns_p3	AATACGAACGTGGCGGAATT	ACACTTTCGTACGACTTGCA	45.0	45.0	58.3	57.8	603 bp	1.0	78.3
F6D16_pgt018-rns_p4	GTTCAAGTCTCTCCGTTCGT	GCATGTGTAAAGCATACCGC	50.0	50.0	57.6	57.9	522 bp	1.0	78.3
F6D16_pgt018-rns_p5	AATACGAACGTGGCGGAATT	TGTGTAAAGCATACCGCCAG	45.0	50.0	58.3	58.3	583 bp	1.0	77.9