Hotspot

psaL-rbcR

Genome · 79585-79635 · 51 bp

Back to Laminaria

Pi 0.0392

MarkerSeek score 67.50

Primer available yes

Label psaL-rbcR

Feature Metadata

label_name: psaL-rbcR
feature_type: igs
region: Genome
length_bp: 51
variable_sites: 2
pi: 0.0392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaL-rbcR_p1	TGGAGGCGTTAGTTTTGCTT	TGAGTCCCAATTATCTGGAA	45.0	40.0	58.0	53.3	191 bp	1.0	43.0
psaL-rbcR_p2	TTGGAGGCGTTAGTTTTGCT	TGAGTCCCAATTATCTGGAA	45.0	40.0	58.0	53.3	192 bp	1.0	43.0
psaL-rbcR_p3	ATTGGTTCATTTGGAGGCGT	TGAGTCCCAATTATCTGGAA	45.0	40.0	58.1	53.3	202 bp	1.0	42.5
psaL-rbcR_p4	TGGAGGCGTTAGTTTTGCTT	TGAGTCCCAATTATCTGGAAA	45.0	38.1	58.0	54.2	191 bp	1.0	42.4
psaL-rbcR_p5	TTGGAGGCGTTAGTTTTGCT	TGAGTCCCAATTATCTGGAAA	45.0	38.1	58.0	54.2	192 bp	1.0	42.4