Hotspot

F6D16_pgt008-dnaK

Genome · 35867-36245 · 379 bp

Back to Laminaria

Pi 0.038

MarkerSeek score 69.70

Primer available yes

Label F6D16_pgt008-dnaK

Feature Metadata

label_name: F6D16_pgt008-dnaK
feature_type: igs
region: Genome
length_bp: 379
variable_sites: 14
pi: 0.038
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
F6D16_pgt008-dnaK_p1	GTTGCGCTCTACCACTGAG	CTGCTTCTGCTAACCCAGAA	57.9	50.0	58.3	57.5	483-494 bp	1.0	70.2
F6D16_pgt008-dnaK_p2	GTTGCGCTCTACCACTGAG	ATTGCTGCTTCTGCTAACCC	57.9	50.0	58.3	58.5	487-498 bp	1.0	69.7
F6D16_pgt008-dnaK_p3	GTTGCGCTCTACCACTGAG	TTGCTGCTTCTGCTAACCC	57.9	52.6	58.3	58.1	486-497 bp	1.0	65.6
F6D16_pgt008-dnaK_p4	CAAGGTTGCGCTCTACCAC	CTGCTTCTGCTAACCCAGAA	57.9	50.0	58.8	57.5	487-498 bp	1.0	65.5
F6D16_pgt008-dnaK_p5	CAAGGTTGCGCTCTACCAC	ATTGCTGCTTCTGCTAACCC	57.9	50.0	58.8	58.5	491-502 bp	1.0	65.1