Hotspot

F6D16_pgt007-ORF1

Genome · 27361-27422 · 62 bp

Back to Laminaria

Pi 0.0645
MarkerSeek score 70.00
Primer available yes
Label F6D16_pgt007-ORF1

Feature Metadata

label_name
F6D16_pgt007-ORF1
feature_type
igs
region
Genome
length_bp
62
variable_sites
4
pi
0.0645
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
F6D16_pgt007-ORF1_p1 AAGGGGGCGGATTGTAAATC AGGTCGAGATGGTCCTGATT 50.0 50.0 57.9 57.8 198 bp 1.0 80.1
F6D16_pgt007-ORF1_p2 GTCACCTAAAGGAGCATGCA AGGTCGAGATGGTCCTGATT 50.0 50.0 57.9 57.8 539 bp 1.0 78.8
F6D16_pgt007-ORF1_p3 TCTTTCCAGCATTGATCGCA AGGTCGAGATGGTCCTGATT 45.0 50.0 57.9 57.8 575 bp 1.0 78.7
F6D16_pgt007-ORF1_p4 AATCCGCTGGTTTTCCTACG AGGTCGAGATGGTCCTGATT 50.0 50.0 58.3 57.8 182 bp 1.0 78.7
F6D16_pgt007-ORF1_p5 TGTCTCAATTGAAGCACGGT AGGTCGAGATGGTCCTGATT 45.0 50.0 57.7 57.8 776 bp 1.0 77.5