Hotspot

F6D16_pgt002-rrns

Genome · 3289-3406 · 118 bp

Back to Laminaria

Pi 0.0254

MarkerSeek score 67.10

Primer available yes

Label F6D16_pgt002-rrns

Feature Metadata

label_name: F6D16_pgt002-rrns
feature_type: igs
region: Genome
length_bp: 118
variable_sites: 3
pi: 0.0254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
F6D16_pgt002-rrns_p1	GCCATCCTGGATTTGAACCA	GACTGGGGTGAAGTCGTAAC	50.0	55.0	58.1	57.9	245 bp	1.0	79.2
F6D16_pgt002-rrns_p2	GCCATCCTGGATTTGAACCA	GTAACAAGGTAGCCGTACGG	50.0	55.0	58.1	58.1	230 bp	1.0	79.2
F6D16_pgt002-rrns_p3	AATGGGCCATCCTGGATTTG	GACTGGGGTGAAGTCGTAAC	50.0	55.0	58.2	57.9	250 bp	1.0	78.7
F6D16_pgt002-rrns_p4	CAAATGGGCCATCCTGGATT	GACTGGGGTGAAGTCGTAAC	50.0	55.0	58.2	57.9	252 bp	1.0	78.7
F6D16_pgt002-rrns_p5	GCCATCCTGGATTTGAACCA	AGGTAAGGCTAGTGACTGGG	50.0	55.0	58.1	58.1	258 bp	1.0	78.7