Hotspot

ycf1

SSC · 122810-128131 · 5322 bp

Back to Lagotis

Pi 0.0152

MarkerSeek score 48.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5322
variable_sites: 119
pi: 0.0152
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAATACCGTCCCTCAACCAC	ACTCGAAACAAGAACTCGGT	55.0	45.0	58.0	57.1	1238-1247 bp	1.0	76.4
ycf1_p2	GTGCCAAGGTTTCAGACAGA	ACTCGAAACAAGAACTCGGT	50.0	45.0	58.0	57.1	1279-1288 bp	1.0	76.2
ycf1_p3	AAGTTCGTTTTCGGGGTGAA	ACTCGAAACAAGAACTCGGT	45.0	45.0	58.0	57.1	1406-1415 bp	1.0	76.2
ycf1_p4	GCAACAGGAATATACGCCCA	ACTCGAAACAAGAACTCGGT	50.0	45.0	58.0	57.1	1115 bp	0.333333	45.9
ycf1_p5	TGCAACAGGAATATACGCCC	ACTCGAAACAAGAACTCGGT	50.0	45.0	58.0	57.1	1116 bp	0.333333	45.9