Hotspot

ndhD-psaC

SSC · 116075-116171 · 97 bp

Back to Lagotis

Pi 0.0893

MarkerSeek score 76.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 97
variable_sites: 13
pi: 0.0893
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TCGTAGTAAAGACAAGATACACT	TAGGGAATGAAACAACCCGC	34.8	50.0	54.6	58.2	169 bp	1.0	46.2
ndhD-psaC_p2	AGGAAAATGATTCGTAGTAAAGACA	TAGGGAATGAAACAACCCGC	32.0	50.0	56.6	58.2	180 bp	1.0	45.8
ndhD-psaC_p3	AGAAAGGAAAATGATTCGTAGT	TAGGGAATGAAACAACCCGC	31.8	50.0	53.5	58.2	184 bp	1.0	45.7
ndhD-psaC_p4	TCGTAGTAAAGACAAGATACACT	AAGAGATGTGAATCCGCCTG	34.8	50.0	54.6	57.7	221 bp	1.0	44.7
ndhD-psaC_p5	AGGAAAATGATTCGTAGTAAAGACA	AAGAGATGTGAATCCGCCTG	32.0	50.0	56.6	57.7	232 bp	1.0	44.5