Hotspot

rpl32-trnL-UAG

SSC · 112264-113137 · 874 bp

Back to Lagotis

Pi 0.0214

MarkerSeek score 61.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 874
variable_sites: 28
pi: 0.0214
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGAAAAGGAAAGGGTGTCG	AAAAATAGCACTGGGCCCAA	50.0	45.0	58.0	58.0	1511-1518 bp	1.0	83.2
rpl32-trnL-UAG_p2	TCAGTCTCTTTCCCCGAGTT	AAAAATAGCACTGGGCCCAA	50.0	45.0	58.0	58.0	2071-2079 bp	1.0	82.7
rpl32-trnL-UAG_p3	TTCAGTCTCTTTCCCCGAGT	AAAAATAGCACTGGGCCCAA	50.0	45.0	58.0	58.0	2072-2080 bp	1.0	82.7
rpl32-trnL-UAG_p4	TCAGTCTCTTTCCCCGAGTT	TGGGCCCAATTATTGTGCTT	50.0	45.0	58.0	58.1	2060-2068 bp	1.0	82.5
rpl32-trnL-UAG_p5	TTCAGTCTCTTTCCCCGAGT	TGGGCCCAATTATTGTGCTT	50.0	45.0	58.0	58.1	2061-2069 bp	1.0	82.5