Hotspot

psaA-ycf3

LSC · 41126-41881 · 756 bp

Back to Lagotis

Pi 0.0106

MarkerSeek score 63.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 12
pi: 0.0106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	947-989 bp	1.0	80.4
psaA-ycf3_p2	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	929-971 bp	1.0	80.4
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	943-985 bp	1.0	78.6
psaA-ycf3_p4	TGAGAAATGACCCGGTTTGG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.1	58.3	945-987 bp	1.0	78.6
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	AGGCTATAGCGCTTACTCCT	50.0	50.0	58.1	57.7	923-965 bp	1.0	78.4