Hotspot

trnG-GCC-trnfM-CAU

LSC · 35812-35979 · 168 bp

Back to Lagotis

Pi 0.012

MarkerSeek score 62.80

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 3
pi: 0.012
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	228-234 bp	1.0	82.3
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	229-235 bp	1.0	82.3
trnG-GCC-trnfM-CAU_p3	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	269-275 bp	1.0	81.5
trnG-GCC-trnfM-CAU_p4	AGAAGATGCGGGTTCGATTC	TGACTTTATCTCTTCGGCGC	50.0	50.0	58.1	58.1	285-291 bp	1.0	81.4
trnG-GCC-trnfM-CAU_p5	GAGAAGATGCGGGTTCGATT	TGACTTTATCTCTTCGGCGC	50.0	50.0	58.1	58.1	286-292 bp	1.0	81.4