Hotspot

petN-psbM

LSC · 28574-29151 · 578 bp

Back to Lagotis

Pi 0.0115

MarkerSeek score 66.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 578
variable_sites: 10
pi: 0.0115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TGCATTTATCGCTACTGCACT	50.0	42.9	58.0	58.1	706-717 bp	1.0	72.9
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TGCATTTATCGCTACTGCACT	50.0	42.9	58.0	58.1	704-715 bp	1.0	72.9
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TGCATTTATCGCTACTGCACT	55.0	42.9	57.9	58.1	703-714 bp	1.0	72.3
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCATTTATCGCTACTGCACT	50.0	42.9	58.2	58.1	758-769 bp	1.0	71.6
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	TGCATTTATCGCTACTGCAC	50.0	45.0	58.0	56.6	704-715 bp	1.0	70.5