Hotspot

atpH-atpI

LSC · 13233-14264 · 1032 bp

Back to Lagotis

Pi 0.00591

MarkerSeek score 64.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1032
variable_sites: 9
pi: 0.00591
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.1	57.9	1107-1117 bp	1.0	80.0
atpH-atpI_p2	ACGGAAGCAGCAGAAATCAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.1	57.9	1106-1116 bp	1.0	80.0
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.8	57.9	1124-1134 bp	1.0	79.1
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1156-1166 bp	1.0	78.9
atpH-atpI_p5	ACGGAAGCAGCAGAAATCAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1155-1165 bp	1.0	78.9