Hotspot

atpB-rbcL

LSC · 53681-54494 · 814 bp

Back to Lagopsis

Pi 0.0033

MarkerSeek score 61.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 814
variable_sites: 4
pi: 0.0033
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GATGATACGCCCCTGGTTTT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	931-937 bp	1.0	78.0
atpB-rbcL_p2	TAGTTGGATGATACGCCCCT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	937-943 bp	1.0	77.7
atpB-rbcL_p3	CCGGACCGATTAGTTGGATG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	947-953 bp	1.0	77.4
atpB-rbcL_p4	TACCGGACCGATTAGTTGGA	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	949-955 bp	1.0	77.3
atpB-rbcL_p5	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	969-975 bp	1.0	77.2