Hotspot

ndhC-trnV-UAC

LSC · 49608-50655 · 1048 bp

Back to Lagopsis

Pi 0.00203
MarkerSeek score 64.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1048
variable_sites
3
pi
0.00203
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAATCCCGGAAAT GAGAAGGTCTACGGTTCGAG 50.0 55.0 57.9 57.2 1094-1180 bp 1.0 76.9
ndhC-trnV-UAC_p2 GGGGCTAAAATCCCGGAAAT CGAGAAGGTCTACGGTTCGA 50.0 55.0 57.9 58.9 1095-1181 bp 1.0 76.2
ndhC-trnV-UAC_p3 TTCTCCGGCCCTTTCCTAAT GAGAAGGTCTACGGTTCGAG 50.0 55.0 58.4 57.2 1115-1201 bp 1.0 74.3
ndhC-trnV-UAC_p4 GGGGCTAAAATCCCGGAAAT AGAAGGTCTACGGTTCGAGC 50.0 55.0 57.9 59.2 1093-1179 bp 1.0 74.0
ndhC-trnV-UAC_p5 TCTCCGGCCCTTTCCTAATA GAGAAGGTCTACGGTTCGAG 50.0 55.0 57.5 57.2 1114-1200 bp 1.0 73.7