Hotspot

trnF-GAA-ndhJ

LSC · 47267-47937 · 671 bp

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Pi 0.00398

MarkerSeek score 61.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 4
pi: 0.00398
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	812-813 bp	1.0	79.8
trnF-GAA-ndhJ_p2	CTACATTGGGAATGGTCGGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	829-830 bp	1.0	79.5
trnF-GAA-ndhJ_p3	TGCTACATTGGGAATGGTCG	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	831-832 bp	1.0	79.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	776-777 bp	1.0	79.4
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GATAATCATCCGCGCCTGAA	55.0	50.0	58.0	58.2	851-852 bp	1.0	78.8