Hotspot

trnK-UUU-rps16

LSC · 4179-5092 · 914 bp

Back to Lagopsis

Pi 0.00365
MarkerSeek score 61.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
914
variable_sites
5
pi
0.00365
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GGTTACGGACGAATTGGTCA TTTCCTTGAAAGGGGTGCTC 50.0 50.0 57.9 58.0 1106-1108 bp 1.0 79.8
trnK-UUU-rps16_p2 TGTAAAAGATTCGAGCCGCA TTTCCTTGAAAGGGGTGCTC 45.0 50.0 57.9 58.0 1073-1075 bp 1.0 79.8
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.2 58.0 1049-1051 bp 1.0 79.1
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.2 58.0 1048-1050 bp 1.0 79.1
trnK-UUU-rps16_p5 ACGGACGAATTGGTCACTTC TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.2 58.0 1102-1104 bp 1.0 78.8