Hotspot

ndhF

SSC · 108307-110529 · 2223 bp

Back to Lagochilus

Pi 0.00475

MarkerSeek score 61.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 37
pi: 0.00475
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGACCCGTTTCTGAAGAGTC	AAAGAGGTAAGGTCGGGTGA	50.0	50.0	56.9	58.0	2363-2372 bp	1.0	72.3
ndhF_p2	AGACCCGTTTCTGAAGAGTC	AAGAGGTAAGGTCGGGTGAA	50.0	50.0	56.9	58.0	2362-2371 bp	1.0	72.3
ndhF_p3	GACCCGTTTCTGAAGAGTCT	AAAGAGGTAAGGTCGGGTGA	50.0	50.0	56.9	58.0	2362-2371 bp	1.0	72.3
ndhF_p4	GACCCGTTTCTGAAGAGTCT	AAGAGGTAAGGTCGGGTGAA	50.0	50.0	56.9	58.0	2361-2370 bp	1.0	72.3
ndhF_p5	GACCCGTTTCTGAAGAGTCT	AGGTCGGGTGAATTGGAAAC	50.0	50.0	56.9	58.1	2353-2362 bp	0.9	67.5