Hotspot

ndhG-ndhI

SSC · 116449-116812 · 364 bp

Back to Lagochilus

Pi 0.0122

MarkerSeek score 65.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 364
variable_sites: 16
pi: 0.0122
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCAAGGAAAAGGCCGAATA	TCAAATTGCTTTAGGTCGGT	50.0	40.0	57.9	55.3	566-586 bp	1.0	63.2
ndhG-ndhI_p2	CCCAAGGAAAAGGCCGAATA	TCAAATTGCTTTAGGTCGGTT	50.0	38.1	57.9	56.1	566-586 bp	1.0	61.7
ndhG-ndhI_p3	CCCAAGGAAAAGGCCGAATA	ATCAAATTGCTTTAGGTCGGT	50.0	38.1	57.9	55.8	567-587 bp	1.0	59.9
ndhG-ndhI_p4	CCCAAGGAAAAGGCCGAATA	AATCAAATTGCTTTAGGTCGGT	50.0	36.4	57.9	56.6	568-588 bp	1.0	57.9
ndhG-ndhI_p5	CCCAAGGAAAAGGCCGAATA	ATCAAATTGCTTTAGGTCGGTT	50.0	36.4	57.9	56.6	567-587 bp	1.0	57.9