Hotspot

trnE-UUC-trnT-CGU

LSC · 30777-31374 · 598 bp

Back to Lagochilus

Pi 0.00519

MarkerSeek score 67.40

Primer available yes

Label trnE-UUC-trnT-CGU

Feature Metadata

label_name: trnE-UUC-trnT-CGU
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 11
pi: 0.00519
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-CGU_p1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	60.0	50.0	58.0	58.0	707-736 bp	1.0	85.0
trnE-UUC-trnT-CGU_p2	CATAGTACCCTACCCCCAGG	CCATGGCGTTACTCTACCAC	60.0	55.0	58.0	58.1	686-715 bp	1.0	84.5
trnE-UUC-trnT-CGU_p3	CATAGTACCCTACCCCCAGG	TGGCGTTACTCTACCACTGA	60.0	50.0	58.0	58.1	683-712 bp	1.0	84.4
trnE-UUC-trnT-CGU_p4	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	60.0	55.0	58.0	58.2	704-733 bp	1.0	83.8
trnE-UUC-trnT-CGU_p5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	672-701 bp	1.0	83.1