Hotspot

petN-psbM

LSC · 28950-29732 · 783 bp

Back to Lagochilus

Pi 0.00813

MarkerSeek score 62.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 783
variable_sites: 20
pi: 0.00813
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCCTACCGCCTTTCTGCTTA	50.0	50.0	58.0	58.4	803-870 bp	1.0	82.3
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCCTACCGCCTTTCTGCTTA	50.0	50.0	58.0	58.4	801-868 bp	1.0	82.3
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TCCTACCGCCTTTCTGCTTA	55.0	50.0	57.9	58.4	800-867 bp	1.0	81.7
petN-psbM_p4	ACTCGTAGTGTGGGGAAGAA	CCTACCGCCTTTCTGCTTAT	50.0	50.0	58.0	57.4	802-869 bp	1.0	80.8
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	CCTACCGCCTTTCTGCTTAT	50.0	50.0	58.0	57.4	800-867 bp	1.0	80.8