Hotspot

ycf1

SSC · 123051-128669 · 5619 bp

Back to Lagerstroemia

Pi 0.0126

MarkerSeek score 47.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5619
variable_sites: 205
pi: 0.0126
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTGGTTGTTCTATCAGCCAG	CCTTTTGGTTCTCCCCGAAA	47.6	50.0	57.9	58.0	393 bp	0.789474	68.7
ycf1_p2	TGTGGTTGTTCTATCAGCCAG	CCTTAAACCTTGGCACAGGT	47.6	50.0	57.9	58.0	514 bp	0.789474	68.2
ycf1_p3	TGTGGTTGTTCTATCAGCCAG	CTCCCCGAAAAAGACGTTCA	47.6	50.0	57.9	58.1	383 bp	0.789474	68.0
ycf1_p4	TGTGGTTGTTCTATCAGCCAG	GTTCTCCCCGAAAAAGACGT	47.6	50.0	57.9	58.1	386 bp	0.789474	67.9
ycf1_p5	TGTGGTTGTTCTATCAGCCAG	ATTTCCCGAATGGTCCGAAG	47.6	50.0	57.9	58.0	661 bp	0.789474	67.0