Hotspot

trnI-GAU-rrn16

IRa · 134970-135213 · 244 bp

Back to Lagerstroemia

Pi 0.0445
MarkerSeek score 63.30
Primer available yes
Label trnI-GAU-rrn16

Feature Metadata

label_name
trnI-GAU-rrn16
feature_type
igs
region
IRa
length_bp
244
variable_sites
23
pi
0.0445
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-rrn16_p1 GGCACAACGACGCAATTATC GCTTGTGACTGGAGTGAAGT 50.0 50.0 58.2 57.8 363-401 bp 1.0 83.1
trnI-GAU-rrn16_p2 CTATCCATGTGGCTGAGAGC GCTTGTGACTGGAGTGAAGT 55.0 50.0 57.8 57.8 395-433 bp 1.0 82.7
trnI-GAU-rrn16_p3 CTATCCATGTGGCTGAGAGC CAAGGTAGCCGTACTGGAAG 55.0 55.0 57.8 57.7 370-408 bp 1.0 82.7
trnI-GAU-rrn16_p4 CGCTGATGAGCACATTGAAC GCTTGTGACTGGAGTGAAGT 50.0 50.0 57.8 57.8 414-452 bp 1.0 82.4
trnI-GAU-rrn16_p5 CGCTGATGAGCACATTGAAC CAAGGTAGCCGTACTGGAAG 50.0 55.0 57.8 57.7 389-427 bp 1.0 82.4