Hotspot

ndhD-psaC

SSC · 116413-116562 · 150 bp

Back to Lagerstroemia

Pi 0.0516

MarkerSeek score 54.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 150
variable_sites: 16
pi: 0.0516
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTTGACCGCTGTACATTGCT	AAACAACTCGAAGCATGGGT	45.0	45.0	58.1	58.0	558 bp	1.0	82.0
ndhD-psaC_p2	TTTGACCGCTGTACATTGCT	GGCATGAAACAACTCGAAGC	45.0	50.0	58.1	58.0	564 bp	1.0	81.9
ndhD-psaC_p3	CCTCCCGCAGTGTACAAAAT	AAACAACTCGAAGCATGGGT	50.0	45.0	58.2	58.0	703 bp	1.0	81.0
ndhD-psaC_p4	CTATAATGTGCCTCCCCGTG	AAACAACTCGAAGCATGGGT	55.0	45.0	57.8	58.0	913 bp	1.0	80.8
ndhD-psaC_p5	GTGGGTGTCTGGTAACCATG	AAACAACTCGAAGCATGGGT	55.0	45.0	58.2	58.0	896 bp	1.0	80.7