Hotspot

petA-psbJ

LSC · 62827-63443 · 617 bp

Back to Lagerstroemia

Pi 0.0274

MarkerSeek score 60.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 41
pi: 0.0274
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	TTCATACTCCGGGTTAGGCT	50.0	50.0	58.0	57.8	761-787 bp	1.0	83.5
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	GTTCATACTCCGGGTTAGGC	50.0	55.0	58.0	57.8	762-788 bp	1.0	83.0
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	TTCATACTCCGGGTTAGGCT	50.0	50.0	57.8	57.8	744-770 bp	1.0	82.7
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	TACTCCGGGTTAGGCTCATC	50.0	55.0	58.0	58.3	757-783 bp	1.0	82.5
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	TCATACTCCGGGTTAGGCTC	50.0	55.0	58.0	58.3	760-786 bp	1.0	82.5