Hotspot

trnS-GCU-trnG-GCC

LSC · 7638-8314 · 677 bp

Back to Lagerstroemia

Pi 0.0189

MarkerSeek score 54.50

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 34
pi: 0.0189
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	738-753 bp	1.0	57.1
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	737-752 bp	1.0	57.1
trnS-GCU-trnG-GCC_p3	GAAAGGCTCGTACAACGGAT	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	761-776 bp	1.0	57.0
trnS-GCU-trnG-GCC_p4	GCTCGTACAACGGATTAGCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.1	55.5	756-771 bp	1.0	56.4
trnS-GCU-trnG-GCC_p5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	792-807 bp	1.0	55.6