Hotspot

rps16-trnQ-UUG

LSC · 5875-6390 · 516 bp

Back to Lagerstroemia

Pi 0.0169
MarkerSeek score 57.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
516
variable_sites
24
pi
0.0169
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TATGTCTGTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 557-677 bp 1.0 86.4
rps16-trnQ-UUG_p2 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 542-662 bp 1.0 85.1
rps16-trnQ-UUG_p3 AGTCGCACGTTGCTTTTTAC GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 546-666 bp 1.0 83.6
rps16-trnQ-UUG_p4 CAAGTCGCACGTTGCTTTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.5 58.1 548-668 bp 1.0 83.0
rps16-trnQ-UUG_p5 TATGTCTGTCAAGTCGCACG GGGTTTTGGTCCCGGTATTC 50.0 55.0 58.0 58.5 578-698 bp 1.0 82.6