Hotspot

ycf1

SSC · 126274-131820 · 5547 bp

Back to Lagenaria

Pi 0.00493

MarkerSeek score 41.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5547
variable_sites: 41
pi: 0.00493
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCCAGGCTTCTGCTATTTC	ATCAAAGGCTCTATGCGTGC	50.0	50.0	57.3	58.7	293 bp	1.0	69.8
ycf1_p2	CCCAGGCTTCTGCTATTTCT	ATCAAAGGCTCTATGCGTGC	50.0	50.0	57.3	58.7	292 bp	1.0	69.7
ycf1_p3	TCCCAGGCTTCTGCTATTTC	GTGCTCAACGACGTAAAACA	50.0	45.0	57.3	57.0	277 bp	1.0	67.4
ycf1_p4	CCCAGGCTTCTGCTATTTCT	GTGCTCAACGACGTAAAACA	50.0	45.0	57.3	57.0	276 bp	1.0	67.3
ycf1_p5	TCCCAGGCTTCTGCTATTTC	AAAGGCTCTATGCGTGCTC	50.0	52.6	57.3	57.9	290 bp	1.0	66.8