Hotspot

petD

LSC · 78633-79843 · 1211 bp

Back to Lagenaria

Pi 0.00165

MarkerSeek score 59.80

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1211
variable_sites: 3
pi: 0.00165
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TTCCCTTCAACTCCACGAAA	TCCCTAGATACACACGTCGT	45.0	50.0	57.0	57.6	1351 bp	1.0	68.8
petD_p2	TTTCCCTTCAACTCCACGAA	TCCCTAGATACACACGTCGT	45.0	50.0	57.0	57.6	1352 bp	1.0	68.8
petD_p3	ATTTCCCTTCAACTCCACGA	TCCCTAGATACACACGTCGT	45.0	50.0	56.8	57.6	1353 bp	1.0	67.0
petD_p4	TCCCTTCAACTCCACGAAAT	TCCCTAGATACACACGTCGT	45.0	50.0	56.8	57.6	1350 bp	1.0	67.0
petD_p5	TTTCCCTTCAACTCCACGAAA	TCCCTAGATACACACGTCGT	42.9	50.0	57.7	57.6	1352 bp	1.0	66.6