Hotspot

ndhF

SSC · 112633-114879 · 2247 bp

Back to Lagenaria

Pi 0.00223

MarkerSeek score 44.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 7
pi: 0.00223
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	GCTCTAGAGCGGGAAATTTTTG	50.0	45.5	58.0	58.0	2921-2922 bp	1.0	64.0
ndhF_p2	ACAGGAACAAGAGGGATCCA	GCTCTAGAGCGGGAAATTTTTG	50.0	45.5	58.0	58.0	2907-2908 bp	1.0	63.8
ndhF_p3	AAGAGGGATCCACCGAAGAA	GCTCTAGAGCGGGAAATTTTTG	50.0	45.5	58.0	58.0	2899-2900 bp	1.0	63.8
ndhF_p4	CTCAGAAACGGAGGAAAGGG	GCTCTAGAGCGGGAAATTTTTG	55.0	45.5	57.9	58.0	2988-2989 bp	1.0	63.3
ndhF_p5	GGGAAAGCGAGGAAGAAACA	GCTCTAGAGCGGGAAATTTTTG	50.0	45.5	58.1	58.0	2968-2969 bp	1.0	63.3