Hotspot

rpl32-trnL-UAG

SSC · 115698-116549 · 852 bp

Back to Lagenaria

Pi 0.0102
MarkerSeek score 63.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
852
variable_sites
13
pi
0.0102
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTGAAAGCTTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.4 58.0 966-972 bp 1.0 77.7
rpl32-trnL-UAG_p2 GGCAGCGTTGAAAGCTTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.4 58.0 967-973 bp 1.0 77.6
rpl32-trnL-UAG_p3 AGCGTTGAAAGCTTTTTCGC AGCGTGTCTACCGATTTCAC 45.0 50.0 58.5 58.0 963-969 bp 1.0 76.9
rpl32-trnL-UAG_p4 GCGTTGAAAGCTTTTTCGCT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.5 58.0 962-968 bp 1.0 76.9
rpl32-trnL-UAG_p5 GCGTTGAAAGCTTTTTCGCT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.5 58.0 963-969 bp 1.0 76.9