Hotspot

ndhF-rpl32

SSC · 114880-115535 · 656 bp

Back to Lagenaria

Pi 0.00712

MarkerSeek score 60.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 656
variable_sites: 7
pi: 0.00712
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAGGTTTTTGGTTGCCGTC	AAAAAGCTTTCAACGCTGCC	50.0	45.0	58.2	58.4	860-862 bp	1.0	76.2
ndhF-rpl32_p2	GTCGAAGGTTTTTGGTTGCC	AAAAAGCTTTCAACGCTGCC	50.0	45.0	58.2	58.4	863-865 bp	1.0	76.2
ndhF-rpl32_p3	GAAGGTTTTTGGTTGCCGTC	AGCGAAAAAGCTTTCAACGC	50.0	45.0	58.2	58.5	864-866 bp	1.0	75.4
ndhF-rpl32_p4	GAAGGTTTTTGGTTGCCGTC	GCGAAAAAGCTTTCAACGCT	50.0	45.0	58.2	58.5	863-865 bp	1.0	75.4
ndhF-rpl32_p5	GTCGAAGGTTTTTGGTTGCC	GCGAAAAAGCTTTCAACGCT	50.0	45.0	58.2	58.5	866-868 bp	1.0	75.4