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rps8-rpl14

LSC · 82683-82830 · 148 bp

Back to Lagenaria

Pi 0.00901

MarkerSeek score 58.20

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 148
variable_sites: 2
pi: 0.00901
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCAGCATTGCGAATAGAGGT	CGATTGCCCGAGAATTGAGA	45.0	50.0	57.0	58.1	266 bp	1.0	72.2
rps8-rpl14_p2	TCAGCATTGCGAATAGAGGT	TTGCCCGAGAATTGAGACAG	45.0	50.0	57.0	57.9	263 bp	1.0	71.9
rps8-rpl14_p3	TCAGCATTGCGAATAGAGGT	GCCCGAGAATTGAGACAGTT	45.0	50.0	57.0	57.9	261 bp	1.0	71.9
rps8-rpl14_p4	TCAGCATTGCGAATAGAGGT	AATTTTTGGTGCGATTGCCC	45.0	45.0	57.0	58.2	277 bp	1.0	71.1
rps8-rpl14_p5	TCAGCATTGCGAATAGAGGTT	CGATTGCCCGAGAATTGAGA	42.9	50.0	57.7	58.1	266 bp	1.0	70.0