Hotspot

trnM-CAU-atpE

LSC · 54338-54517 · 180 bp

Back to Lagenaria

Pi 0.0333

MarkerSeek score 61.30

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 9
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GATGTGATGTGATAGGGCCC	CTCTCAGACGAGCTAGGACA	55.0	55.0	57.8	58.0	353 bp	1.0	80.3
trnM-CAU-atpE_p2	GATGTGATGTGATAGGGCCC	CGAGCTAGGACACGAGTAGA	55.0	55.0	57.8	58.1	345 bp	1.0	80.1
trnM-CAU-atpE_p3	GATGTGATGTGATAGGGCCC	GAGGCTATCAATGGCGTACC	55.0	55.0	57.8	58.2	327 bp	1.0	79.0
trnM-CAU-atpE_p4	GATGTGATGTGATAGGGCCC	TCTCAGACGAGCTAGGACAC	55.0	55.0	57.8	58.3	352 bp	1.0	78.4
trnM-CAU-atpE_p5	GATGTGATGTGATAGGGCCC	CTCAGGGCAAGAGACAAACA	55.0	50.0	57.8	57.7	389 bp	1.0	78.2