Hotspot

trnF-GAA-ndhJ

LSC · 50291-50993 · 703 bp

Back to Lagenaria

Pi 0.0038

MarkerSeek score 57.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 4
pi: 0.0038
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGGTAGGATGGCC	50.0	55.0	58.1	58.2	812 bp	1.0	78.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGTAGGATGGCCCTTACGTA	50.0	55.0	58.1	58.3	804 bp	1.0	77.7
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGGTAGGATGGC	50.0	50.0	58.1	57.5	813 bp	1.0	76.0
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGCCTGAAAGTTGGGTAGG	50.0	50.0	58.1	57.5	818 bp	1.0	76.0
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGCCTGAAAGTTGGGTAGGA	50.0	50.0	58.1	58.6	817 bp	1.0	75.6