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trnC-GCA-petN

LSC · 29188-29770 · 583 bp

Back to Lagenaria

Pi 0.00802

MarkerSeek score 51.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 7
pi: 0.00802
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	756-757 bp	1.0	77.6
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	699-700 bp	1.0	77.5
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	TCTTCCCCATACTACGAGGG	45.0	55.0	57.9	57.6	746-747 bp	1.0	77.4
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	689-690 bp	1.0	77.3
trnC-GCA-petN_p5	TGCAAATCCCTTTTTCCCCA	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	711-712 bp	1.0	77.2