Hotspot

trnG-UCC-trnR-UCU

LSC · 10497-10736 · 240 bp

Back to Lagenaria

Pi 0.00575

MarkerSeek score 59.50

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 2
pi: 0.00575
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	308-320 bp	1.0	66.9
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	311-323 bp	1.0	66.7
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	314-326 bp	1.0	66.2
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	317-329 bp	1.0	65.9
trnG-UCC-trnR-UCU_p5	ACCAAAGTTTCACGTTCGGT	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.3	56.9	378-390 bp	1.0	64.4