Hotspot

psbI-trnS-GCU

LSC · 8752-8856 · 105 bp

Back to Lagenaria

Pi 0.019

MarkerSeek score 60.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 105
variable_sites: 3
pi: 0.019
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TCCTATCTAATGACCCGGGAC	TAAAGCGGCGGATTGCTAAT	52.4	45.0	58.1	58.0	217 bp	1.0	71.9
psbI-trnS-GCU_p2	AATCCGGGGCGTGAAGAATA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	59.2	58.0	194 bp	1.0	71.6
psbI-trnS-GCU_p3	ATCCGGGGCGTGAAGAATAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	59.2	58.0	193 bp	1.0	71.6
psbI-trnS-GCU_p4	TAATCCGGGGCGTGAAGAAT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	59.2	58.0	195 bp	1.0	71.6
psbI-trnS-GCU_p5	TCCTATCTAATGACCCGGGA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	56.6	58.0	217 bp	1.0	69.7