Hotspot

trnQ-UUG-psbK

LSC · 7700-8075 · 376 bp

Back to Lagenaria

Pi 0

MarkerSeek score 53.00

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	AATACTCTGGGACGGAAGGA	GGCAATTTGGCGACGAAAAA	50.0	45.0	57.5	58.5	532-534 bp	1.0	71.9
trnQ-UUG-psbK_p2	TACTCTGGGACGGAAGGATT	GGCAATTTGGCGACGAAAAA	50.0	45.0	57.5	58.5	530-532 bp	1.0	71.9
trnQ-UUG-psbK_p3	GACCAAAACCCGTTGCCTTA	GGCAATTTGGCGACGAAAAA	50.0	45.0	58.7	58.5	492-494 bp	1.0	70.8
trnQ-UUG-psbK_p4	ATACTCTGGGACGGAAGGAT	GGCAATTTGGCGACGAAAAA	50.0	45.0	57.2	58.5	531-533 bp	1.0	70.0
trnQ-UUG-psbK_p5	ACTCTGGGACGGAAGGATTC	GGCAATTTGGCGACGAAAAA	55.0	45.0	58.8	58.5	529-531 bp	1.0	69.8