Hotspot

ycf2

IRa · 151656-158534 · 6879 bp

Back to Lagarosiphon

Pi 0.0369

MarkerSeek score 65.30

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6879
variable_sites: 334
pi: 0.0369
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CAAAGATCTTGAGCGACCGA	AGTTCTCCACCCTAACGACA	50.0	50.0	58.0	58.0	1330-1354 bp	0.666667	73.2
ycf2_p2	TCCGGCAGAACAACTCAAAA	AGTTCTCCACCCTAACGACA	45.0	50.0	58.0	58.0	1310-1334 bp	0.666667	72.9
ycf2_p3	GTGCTACAAGATCTCGTGCA	AGTTCTCCACCCTAACGACA	50.0	50.0	58.0	58.0	981 bp	0.333333	53.3
ycf2_p4	AGATCTCGTGCATTGGAACC	AGTTCTCCACCCTAACGACA	50.0	50.0	58.0	58.0	973 bp	0.333333	53.1
ycf2_p5	GTGCTACAAGATCTCGTGCA	CCGCTTATGGGGTCAAATCA	50.0	50.0	58.0	57.9	1242 bp	0.333333	52.9