Hotspot

petA-psbJ

LSC · 67865-68807 · 943 bp

Back to Lagarosiphon

Pi 0.0539

MarkerSeek score 80.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 74
pi: 0.0539
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGGCGTCAAGTGGTTGATAT	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	1897-1907 bp	1.0	84.3
petA-psbJ_p2	GGGCGTCAAGTGGTTGATAT	TGGACTAGCTGTACCTACCG	50.0	55.0	58.0	58.0	1666-1671 bp	0.666667	70.9
petA-psbJ_p3	GGGCGTCAAGTGGTTGATAT	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1665-1670 bp	0.666667	70.9
petA-psbJ_p4	CAAGATCCATTACGCGTCCA	TGGACTAGCTGTACCTACCG	50.0	55.0	58.1	58.0	1528 bp	0.333333	52.4
petA-psbJ_p5	CAAGATCCATTACGCGTCCA	GGACTAGCTGTACCTACCGT	50.0	55.0	58.1	58.0	1527 bp	0.333333	52.4