Hotspot

trnD-GUC-trnY-GUA

LSC · 33058-33437 · 380 bp

Back to Lagarosiphon

Pi 0.0563

MarkerSeek score 81.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 31
pi: 0.0563
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	465-474 bp	1.0	80.9
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	464-473 bp	1.0	80.7
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.5	58.4	498-507 bp	1.0	79.9
trnD-GUC-trnY-GUA_p4	ATCCTAGTTCGGGACTGACG	GGGGACGGACTGTAAATTCG	55.0	55.0	58.6	58.4	518-527 bp	1.0	79.0
trnD-GUC-trnY-GUA_p5	ATCCTAGTTCGGGACTGACG	GGGACGGACTGTAAATTCGT	55.0	50.0	58.6	57.6	517-526 bp	1.0	78.9