Hotspot

ycf1

SSC · 129077-134701 · 5625 bp

Back to Lafoensia

Pi 0.00533

MarkerSeek score 42.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 30
pi: 0.00533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTCGTCTTCTGGAACTGCA	CCTCATCGCGGAAAGATCAA	50.0	50.0	58.1	58.1	208 bp	1.0	80.0
ycf1_p2	TGTTCGTCTTCTGGAACTGC	CCTCATCGCGGAAAGATCAA	50.0	50.0	58.1	58.1	209 bp	1.0	80.0
ycf1_p3	GTTCGTCTTCTGGAACTGCA	TTTTGACTGGACCTCATCGC	50.0	50.0	58.1	58.2	219 bp	1.0	78.9
ycf1_p4	TGTTCGTCTTCTGGAACTGC	TTTTGACTGGACCTCATCGC	50.0	50.0	58.1	58.2	220 bp	1.0	78.8
ycf1_p5	GTTCGTCTTCTGGAACTGCA	GACCTCATCGCGGAAAGATC	50.0	55.0	58.1	58.5	210 bp	1.0	76.4